DEMONSTRATION OF MITOCHONDRIAL ATPASE ACTIVITY IN FORMALIN-FIXED COLONIC EPITHELIAL CELLS

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Demonstration of Mitochondrial Atpase Activity in Formalin-fixed Colonic Epithelial Cells

The intracel lular dis t r ibut ion of adenosine triphosphatase (ATPase) activity has been extensively studied by means of two histochemical methods in which the inorganic phosphate (Pi) produced upon the enzymat ic hydrolysis of adenosine triphosphate (ATP) is t r apped as an insoluble salt (26, 40). The appl icat ion of these methods in l ight microscope studies on a var iety of unfixed tissu...

متن کامل

The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labelled antibody.

A method is described for the demonstration of specific immunoglobulin in plasma cells and other lymphoid cells in sections taken from routine surgical histology specimens which have been formalin fixed and paraffin embedded. An indirect sandwich technique was employed using specific rabbit antihuman immunoglobulin antisera (anti-K, L, G, A, and M) and a swine antirabbit serum Ig G, conjugated ...

متن کامل

A Method to Evaluate Genome-Wide Methylation in Archival Formalin-Fixed, Paraffin-Embedded Ovarian Epithelial Cells

BACKGROUND The use of DNA from archival formalin and paraffin embedded (FFPE) tissue for genetic and epigenetic analyses may be problematic, since the DNA is often degraded and only limited amounts may be available. Thus, it is currently not known whether genome-wide methylation can be reliably assessed in DNA from archival FFPE tissue. METHODOLOGY/PRINCIPAL FINDINGS Ovarian tissues, which we...

متن کامل

Quantitative analysis of mast cells in benign and malignant colonic lesions: immunohistochemical study on formalin-fixed, paraffin-embedded tissues

Background: Comparison of the number of mast cells in the active stage and that in remission in the same patients with ulcerative colitis with immunohistochemical staining remains to be elucidated, and analysis of the number of mast cells in benign and malignant colonic lesions is insufficient. Methods: Using immunohistochemical methods, morphological examinations of mast cells were undertaken ...

متن کامل

Electron Microscopic Radioautopgraphic Study on Mitochondrial Protein Synthesis in Colonic Epithelial Cells of Aging Mice

For the purpose of studying the aging changes of macromolecular synthesis in the colonic cells of experimental animals, we studied 10 groups of aging mice during aging from fetal day 19 to postnatal month 24. They were injected with 3H-leucine, a precursor for protein synthesis, sacrificed and the colonic tissues were processed for light and electron microscopic radioautography. On many radioau...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 1963

ISSN: 1540-8140,0021-9525

DOI: 10.1083/jcb.19.3.647